Integrated Biomarker Discovery And T Cell Analysis

Having the ability to reliably profile individual T cell function is a crucial part of therapeutic development. Fields such as cancer immunotherapy, vaccine development, infectious diseases, autoimmune disease and organ transplantation, have an urgent need for new biomarkers to identify the most potent T cells. The Bruker Ecosystem for Functional T Cell Profiling, including the IsoSpark™ and Beacon^® platforms, offers a solution to gaining a deeper understanding of T cell biology with a suite of integrated tools.

In this Application Note we outline:

• Bruker’s ecosystem for functional T cell profiling using the IsoSpark and Beacon.
• The IsoSpark’s ability to measure highly multiplexed cytokine secretions from single cells.
• Workflows to identify correlative biomarkers on the IsoSpark for focused analysis on the Beacon.
• How the Beacon uses optofluidic technology to evaluate cytokine secretion and cytotoxicity, with the ability to recover cells of interest.
• Workflows to create therapies using the Beacon and analyzing potency of these novel therapeutics on the IsoSpark.