Multimode Microplate Reader: Infinite® F500

The Infinite F500 is Tecan’s most sensitive filter-based multimode microplate reader, to provide a multifunctional and modular system which offers excellent levels of sensitivity for fluorescence- and luminescence-based assays.

The Infinite F500 is compatible with a wide variety of microplates, from 6- to 1,536-wells, including standard and low volume plate formats and Tecan’s patent pending NanoQuant PlateTM. Its high speed, on-the-fly measurement capabilities ensure maximum throughput, allowing complete reading of a 1,536-well plate in under 30 seconds. It supports a broad range of measurement modes and functions, allowing straightforward application.

Ideal for advanced assays in research, drug discovery and routine laboratory applications

The Infinite F500 supports a broad range of microplate-based detection systems, for applications including  biomolecular screening, enzymatic assays, reporter gene assays and molecular binding studies – as well as TR-FRET assays and flash luminescence measurements using the optional injectors – and is ideal for biotechnology and screening applications. It offers outstanding sensitivity and speed – particular for FI, FP, FRET and TR-FRET assays – due to its separate excitation and emission filter slide design and ultra-fast filter switching. Numerous exchangeable filters are available, allowing the user to quickly and easily adapt the instrument to suit a wide range of biochemical and ratiometric measurements.

Features

• High measurement speed and robotic compatibility
• NanoQuant Plate™ compatibility: DNA quantification in 2 μl samples
• z-Optimization: for better results in low volumes
• Programmable filter slides

Supports many different applications and assay technologies:

• TR-FRET-based assays: e.g. HTRF® (Cisbio bioassays, France), LanthaScreen® (Invitrogen).
• FRET-based assays: e.g. Z`-LYTE® (Invitrogen) to study cellular protein kinases, GeneBLAzer® (Invitrogen) Reporter Gene assays.
• Glow luminescence: cell-based & reporter gene assays
• Flash luminescence: e.g. dual luciferase assay (DLR®) and various CA2+ detection assays if optional injectors are installed
• Fluorescence polarization: e.g. Predictor™ hERG Fluorescence Polarization Assay (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Red (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green (Invitrogen), Transcreener® ADP Assay (BellBrook Labs)

Validated by Invitrogen for the following Invitrogen assays:

• LanthaScreen®
• Adapta® Universal Kinase Assay
• Omnia®
• Z’-LYTE®
• GeneBLAzer®
• PolarScreen™
• Predictor™ hERG Fluorescence Polarization Assay