Improving Aggregate Removal From mAb Feed Using Cation Exchange Chromatography

Ever increasing performance demands in protein purification require more selective chromatography methods to effectively remove aggregates and other impurities. At high titer levels, aggregates often present a unique purification challenge. One emerging approach to address this challenge is the use of new, smaller-particle chromatography resins that are optimized for high resolution and capacity. Such resins can be particularly productive in challenging situations and during final polishing steps (He et al. 2010). Nuvia™ HR-S is a high-resolution resin. It features a hydrophilic polymer matrix with an open-pore structure designed for fast and efficient mass transfer and superior flow properties at high flow rates. In this case study, the performance of Nuvia HR-S was compared to that of a high-resolution agarose-based resin.