Improved Sensitivity In Flow Cytometry By In-Line Pre-Enrichment Of CAR T Cells And Antigenspecific T Cells On The MACSQuant® Analyzer

Detecting rare immune cell populations remains a persistent limitation in flow cytometry, especially when monitoring CAR T cells or antigen‑specific T cells present at extremely low frequencies. Explore how integrating magnetic pre‑enrichment directly into the flow cytometry workflow can dramatically extend analytical sensitivity without sacrificing phenotypic detail. By combining targeted labeling strategies with automated enrichment, rare cell populations become reliably detectable at levels well below the traditional limits of standard cytometry. The approach enables clearer separation of true target cells from background noise, improves quantification across a wide dynamic range, and supports more confident determination of detection and quantitation limits. Together, these advances point to a more robust strategy for immune monitoring, long‑term cell therapy tracking, and detailed characterization of low‑frequency T cell responses.

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