Improve CD34+ Yields For A Robust, Reproducible Feeder-Free Human iNK Differentiation Process

By Eli Perr, Yiao Wang and Xi Lu

Current sources of Natural Killer (NK) cells such as umbilical cord (UCB) or Peripheral blood (PB) cannot easily expand to clinically relevant numbers and are heterogeneous in cell composition. Human iPSCs-derived NKs (iNKs) may overcome these obstacles by providing a uniform and potentially unlimited source of NKs for allogeneic cell therapy. The results of a study into optimizing CD34+yield during differentiation of iPSCs to HSCs, showed significant improvement in the yield of iNKs in a feeder-free differentiation process. Explore a combination of methods that demonstrate a complete workflow for obtaining highly cytotoxic NK cells derived from iPSCs appropriate for the clinic.