Impact Of A Chemically Defined Medium On Vero Cells Growth And Productivity Essential In Vaccine Manufacturing

Source: Sartorius

By Roni Hazan Brill, David Fiorentini, Ber van der Wijden, Bella Monica, Gerco van Eikenhorst, Emilie Rodrigues, Yvonne E. Thomassen, Adrien Lugari, Maverick Lau, √Črica A. Schulze

The Vero cell line is now one of the most commonly used cell line for biopharmaceuticals production such as cell culture-based viral vaccines. An example is the successful production of both live (rotavirus, smallpox) and inactivated (poliovirus) viral vaccines. Reasons for the extensive use of the Vero cell line are the consistently high viral yields and relatively easy adaptation for growth in bioreactors on microcarriers, thus allowing greater vaccine purity and as well as quantity.

Currently available media to cultivate Vero cells contain undefined plant hydrolysates or animal-derived raw materials such as yeastolates. These undefined materials lead to variability in media performance, a result of lot-to-lot variations, increased potential for contamination, as well as inconsistency when scaling up for commercial processes. Sartorius has introduced 4Cell® NutriVero™ Flex 10 Medium, a chemically defined (CD), serum-free, animal origin-free, and hydrolysate-free medium optimized for both 2D monolayer and 3D microcarrier suspension culturing of Vero cells. It reduces process variability and enhances process safety, providing greater predictability during scale-up and improved virus productivity achieving comparable performance to hydrolysate-containing, serum-free media.

Continue reading to see how the chemically defined 4Cell® NutriVero™ Flex 10 provides the ultimate environment for improved Vero cell viability and yield, as well as high virus production while maintaining a completely defined animal component-free manufacturing process.

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