Identification Of Conjugation Sites In An Antibody Drug Conjugate

By Yulan Bian

Precise identification of conjugation sites is critical to understanding and optimizing antibody–drug conjugates. A streamlined workflow integrates automated protein sample preparation with high-resolution LC/Q-TOF mass spectrometry to map lysine-linked modifications with confidence. In a T-DM1 case study, 26 conjugation sites are identified, supported by strong mass accuracy and consistent reproducibility across runs. The approach highlights how coordinated instrumentation and data analysis software can simplify complex characterization challenges while maintaining analytical rigor. For teams working to accelerate biologics development and improve confidence in ADC characterization, this workflow offers a practical path forward.

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