Product/Service

Human Leptin ELISA Kit

Source: Linco Research, Inc.
This product is a two-site immunoassay using a high affinity polyclonal capture antibody and a monoclonal detecting antibody
Features:
  • Human Leptin
  • GLP-1 (Active)
  • Amylin
  • Total Amylin
  • ASSAY CONDITIONS: 3 1/2 hour, room temperature incubation. Sample volume: 50 µl serum, plasma, or tissue culture media.
  • All components are included (96-well antibody coated plate, standards, biotinylated antibody, quality controls, streptavidin-alkaline phosphatase, pNPP, wash buffer and assay buffer) to complete a 96-well plate ELISA
  • Developed to measure total human leptin in serum, plasma or tissue culture media.

This product is a two-site immunoassay using a high affinity polyclonal capture antibody and a monoclonal detecting antibody. Signal is derived by monitoring (405 nm) alkaline phosphatase activity in the presence of the substrate p-nitrophenyl phosphate. This assay produces answers identical to our gold standard Human Leptin RIA and offers an alternative for laboratories interested in reducing use of radioisotopes yet maintaining data consistency to previously published RIA results.

Linco Research, Inc., 14 Research Park Dr., St. Charles, MO 63304. Tel: 636-441-8400; Fax: 636-441-8050.