Human Induced Pluripotent Stem Cell Aggregate Expansion In Stirred-Tank Bioreactors Using SciVario^® Twin Bioprocess Controller

By Jorge L. Escobar Ivirico and Ma Sha

The implementation of cell-based therapies is often challenged by limited cell availability and process scalability. Human Induced Pluripotent Stem Cell (hiPSC) are an attractive cell platform choice for developing and manufacturing off-the-shelf cell products, as they are amenable to gene modification and can be expanded limitlessly. By isolating and expanding a single hiPSC colony from a heterogeneous culture, it is possible to obtain a more consistent and high quality hiPSC population (or inoculum) with identical genetic characteristics to the parent cells. This can also reduce variability within cultures and provide consistency in pluripotency.