Human Growth Factor Concentrate: Physiologix™ XF SR

The Physiologix™ Advantage

• Streamline Regulatory Compliance: GMP and completely xeno-free, Physiologix can simplify your transition from development to commercialization.
• Proven Serum Replacement: Drive desirable CQAs at superior or equivalent levels to FBS and human serum in both T cells and stem cells.
• Consistent Quality: Proprietary manufacturing process and rigorous quality control yield documented consistency across lots.

Take Your Cells Farther

Replacing serum with xeno-free, GMP Physiologix brings consistent quality, increases cell performance, and streamlines regulatory to your cell culture research.

Enhances Transduction Efficiency

Physiologix promotes viral entry and enhances lentiviral-mediated gene expression. As shown, media supplemented with 2% Physiologix (OP) demonstrate significantly enhanced transduction efficiency measured as % expression of red fluorescent protein (RFP) (compared to 5% human serum (OH)), resulting in a greater proportion of transfected cells and, in turn, a higher potency final product at lower manufacturing costs. Data shown represents 3 donors using Optimizer™ media in a G-Rex® model.

Greater Efficacy With More CAR+ Cells

Physiologix can improve the therapeutic potential and in-vivo efficacy of CAR T-cell therapy by promoting a more potent antitumor response. In data published by UPenn and Nucleus Biologics, anti-GD2 CAR-T cells expanded in OpTmizer™ + 2% Physiologix (OP) demonstrated significantly superior tumor killing in an in-vivo solid tumor neuroblastoma model when compared to CAR-T cells grown in OpTmizer + 5% human serum (OH). Data shows quantification of tumor burden in total flux (photons/second) by bioluminescence imaging on days 15 (3×10⁶ CAR-T cell dose) and 19 (0.75×10⁶ CAR-T cell dose).

Maintains Cell Health and Desirable Characteristics

Physiologix maintains cell health, viability, and desired phenotypes equivalent to human serum. Media supplemented with 2% Physiologix (OP) in a 7-day analysis in a G-Rex® model demonstrated strong proliferation and population doubling (data not shown) when compared to media + 5% human serum (OH). Additionally, desired naïve and central memory phenotypes are preferentially maintained as with human serum. Data shown represents average of 3 donors ±SD using Optimizer™ media.

Applicability in Stem Cell Models

Physiologix can support proliferation in other cell models such as mesenchymal stem cells (MSCs). DMEM/F-12 media supplemented with Physiologix (PX) have increased cell proliferation rates at earlier time points (compared to FBS) resulting in shortened protocols, which could reduce labor and consumable costs, lower energy consumption, and accelerate therapy delivery without affecting the quality of the cells.

Specifications