By Amanda Suttle, John Longsworth, and Ma Sha, Eppendorf, Inc.,
To scale upstream bioprocesses, an effective scale-up strategy is required to ensure the reproducibility of both cell culture growth and batch yields at large working volumes, ideally with little or no additional optimization. The Constant Power approach is a successful method for matching bioreactor yields at different scales. This study details the use of this new Scale Up Assist feature of the BioFlo 720 Bioreactor Control System to scale CHO batch cultures from 3 L to 10 L BioBLU® Single-Use Vessels and from the 10 L to 50 L Single Use Bioreactor (SUB) bag. Using a built-in constant power software algorithm, the Scale Up Assist specified what agitation and gassing set points to use for different vessel sizes.
We cultivated Chinese Hamster Ovary cells (CHO) using BioBLU Single-Use Vessels and a Thermo ScientificTM HyPerformaTM 50 L SUB. Our results demonstrated that the parameters provided by the new Scale Up Assist software resulted in similar cell growth and monoclonal antibody (mAb) production yields for all three vessel sizes. Adoption of the controller’s Scale Up Assist function can decrease process development time while also helping to ensure consistent mAb yields across vessel sizes and bioreactor platforms.