How Does GS piggyBac® Transposon Technology Work?

Traditional cell line development has often relied on random integration to introduce expression vectors into the host cell genome. In this video, Lonza’s Dr. Peter O’Callaghan, Senior Director and Head of Expression System Sciences, Licensing, explains how GS piggyBac® transposon technology takes a more targeted approach to support high and stable protein expression.

The discussion explores how Lonza’s engineered GS piggyBac® transposase is designed to integrate expression cargo into accessible regions of the CHO genome associated with high and stable expression. By combining the transposase with optimized GS expression vectors, the technology can be incorporated into established workflows for generating stable pools and clonal cell lines.

Peter also highlights performance benefits observed across a range of product types, including faster stable pool recovery and reported titer improvements compared with traditional random integration approaches. The technology is designed to integrate seamlessly with existing GS Gene Expression System® workflows, with the same host cell lines, expression vectors, media, and feeds supporting development from transient expression through stable pools and clonal cell lines.

Watch the full video to learn how GS piggyBac® technology can simplify cell line development while supporting faster workflows, high expression, and consistency across the product development lifecycle.