Higher Throughput Intact Mass Confirmation And Impurity Screening Of GLP-1 Analogues Using Multi-Reflecting TOF Technology And INTACT Mass 1.9 Application

By Jonathan Fox, Scott J Berger, Laetitia Denbigh, and Ying Qing Yu

Analyzing glucagon-like peptide 1 (GLP-1) analogues requires speed, precision, and dependable impurity detection. A higher-throughput intact mass workflow combines the ACQUITY™ Premier UPLC™ System, Xevo™ MRT Mass Spectrometer, and waters_connect™ INTACT Mass 1.9 Application to simplify this complex analysis. The integrated approach confirms main species mass, calculates purity automatically, and assigns targeted impurities in under one minute per sample. High-resolution mass data and BayesSpray deconvolution provide consistent sub-ppm mass accuracy while detecting impurities at relative abundances as low as 0.03%. By reducing manual review and improving consistency, the workflow supports scalable screening and quality control across peptide development and commercialization.

Access the full application note to see how rapid, automated intact mass analysis can strengthen process control and product integrity for GLP-1s and other synthetic peptides.