High-Throughput Multi-Capillary SDS Gel Electrophoresis Of Proteins

By Roy Estrada, Zuzana Demianova, Tingting Li, Marcia Santos, Andras Guttman, and Sahana Mollah

The SCIEX BioPhase 8800 system utilized a multicapillary format that enables characterization to be performed on multiple samples in parallel. In this work, SDS-CGE analysis of multiple samples simultaneously to accelerate overall analysis time and potentially shorten product development workflows will be demonstrated. The BioPhase 8800 system additionally allows for analysis using UV and fluorescent detection in the same sequence, further reducing method development time.

This study evaluates a multicapillary electrophoresis system (BioPhase 8800 system) in respect to migration time and the corrected peak area% reproducibility in SDS-CGE of a monoclonal antibody, and compares the results to a single capillary electrophoresis unit (PA 800 Plus). The SCIEX SDS-MW gel buffer was used in all experiments as separation matrix.

In this technical note, high throughput SDS-CGE analysis is demonstrated using the new BioPhase 8800 system and the results are compared to the single capillary PA 800 Plus pharmaceutical analysis system in respect to migration time, peak area% reproducibility, resolution and throughput.

Discover how the multi-capillary system can be used to accelerate development of sensitive, high-throughput analytical methods, while potentially decreasing time to market for biologics.