High-Throughput 90-Minute ELISA: 384-Well SimpleStep ELISA® With The PHERAstar® FSX

By Jamie Campbell, Director of Custom Solutions EMEA – Abcam, and Oliver Carney, BMG LABTECH

This content is brought to you by Abcam, a Danaher Operating Company.

More than forty years after its introduction, the enzyme-linked immunosorbent assay (ELISA) remains one of the most trusted and widely used methods for detecting and quantifying proteins and other analytes in a variety of biological samples. It is often referred to as the gold standard in such applications. However, traditional ELISA techniques involve multiple washing steps, lengthy processing times, and large sample volumes, which can make adapting them to high-throughput workflows difficult.

Abcam’s SimpleStep ELISA® technology simplifies this process by offering a semi-homogeneous format with a streamlined, single-wash protocol that takes just 90 minutes. For higher throughput needs, the SimpleStep ELISA® can be scaled down to a 384-well format, allowing up to four times the number of samples to be processed in the same amount of time. When paired with the BMG LABTECH PHERAstar® FSX, this system achieves higher throughput while maintaining high sensitivity, with each well being read in less than a second. The accompanying BMG LABTECH MARS software enables the automatic generation of quantitative standard curves as well as the quantitation of unknown samples. Continue reading to explore how the SimpleStep ELISA® in a 384-well format, combined with the PHERAstar® FSX, can be utilized to measure Human IgG and human IFN gamma, which are key markers in pharmacokinetic studies and the testing of immunotherapeutic drug safety.