High-Resolution LC-MS/MS Solution For Improved Quantification Of Peptides In A Complex Matrix

By Shane Needham, Eshani Nandita, Lei Xiong, Elliott Jones, Zoe Zhang, and Kerstin Pohl

Traditional workflows for quantitative bioanalyses, such as immunological assays, have been displaced by LC-MS/MS analysis on a triple quadrupole mass spectrometer. Immunoassays often lack selectivity, specificity, and have a limited linear dynamic range.

In this application note, explore how improved selectivity and greater sensitivity were achieved for peptide quantitfication in a complex matrix using the ZenoTOF 7600 system featuring the Zeno trap. In addition, enhanced sensitivity for peptide quantification was achievable using the Zeno trap to improve the duty cycle versus traditional time-of-flight systems.