High-Resolution Characterization Of Both sgRNA And Cas9 mRNA In A Single Analysis For CRISPR/Cas9 Applications

By Mario A. Pulido, Mervin Gutierrez, Fang Wang, Jane Luo and Marcia Santos

Overcome the challenges of accurately characterizing and quantifying differently sized RNA molecules, impurities, and degraded RNA species in vaccines. The RNA 9000 Purity & Integrity Kit improves your characterization of both RNA integrity in the final product and RNA fragmentation with an analytical kit-based solution that can separate single-stranded RNA products in the range of 50 to 9,000 bases.

Key features of the kits include:

• Ability to acquire high-resolution data for RNA fragments ranging from 50 to 9,000 bases using the RNA 9000 Purity & Integrity Kit
• Excellent %CV, < 2%, for the corrected peak area% of the main product of the Cas9 mRNA component of the CRISPR/Cas9 gene editing system
• Ready-to-use, validated kit and methods enable efficient RNA fragment characterization using the high-throughput BioPhase 8800 system