High-Density Escherichia Coli Fed-Batch Fermentation Using The SciVario® Twin Bioreactor Control System

By Robert Glaser, Anne Niehus, and Ma Sha

In upstream bioprocessing, the feed strategy is an important parameter to achieve high cell densities. Experts have conducted a study which optimized high-cell-density fed-batch fermentation of Escherichia coli (E. coli) using the SciVario twin bioreactor control system equipped with a 1 L glass bioreactor. Improving the medium composition and feeding strategy resulted in a more than two-fold increase of the optical density (OD600) compared to a previous study using the same strain.

These experiments highlight SciVario twin’s capabilities to control complex processes achieving high cell densities. Check out the information gathered from this study that has become a starting point for further optimization of high biomass based E. coli bioproduction. The presented experiments provide preliminary results addressing feasibility without extensive optimization and thus do not represent the maximum fermentation potential. However, they serve as a starting point for further process optimization.