HCPure™: A Fit For Purpose Host Cell Protein Clearance Solution In E. Coli Workflows

mAb biologics have been released for a wide variety of conditions including cancer treatments, rheumatology, hematology and infectious diseases. These mAbs and processes to develop them will only become more necessary as the global population ages and chronic illnesses become more prevalent. Removal of host cell proteins and final product purity are critical to effective therapeutic outcomes. By failing to effectively clear the final sample of HCPs, a range of negative outcomes can occur, from changes in therapeutic effectiveness to immunogenic responses in the patient. Mixed mode chromatography can streamline the polishing process by utilizing both ionic exchange and hydrophobic interaction binding methods.

Explore both the binding properties of HCPure™ ligand as well as demonstrating that HCPure™ can act as an effective single polishing step when purifying Vκ in E. coli feedstocks.