Got Purification Troubles? Use Cation Exchange Chromatography For High Yields In Vaccine Production

For large-scale vaccine production, efficient purification of antigen proteins is a critical challenge, especially when standard affinity-based methods limit yields. Learn how Cation Exchange Chromatography (CEX) can be highly effective in purifying SARS-CoV-2 spike ectodomain proteins, offering a non-affinity solution for high-yield, population-scale manufacturing.

A study investigated a purification workflow using Nuvia HR-S Resin for the final chromatography step. This strong cation exchanger, featuring 50 µm pores, excels at minimizing on-column protein aggregates, which leads to high purity. The process showed superior performance for the ancestral spike protein, achieving >80% recovery with 80% purity and very low aggregation levels. The CEX bind-and-elute method sharply separates monomers from aggregates in a single step.

Discover the full purification workflow, including buffer details and analytical results, to optimize your vaccine production process.