Got Purification Troubles? Minimize On-Column Aggregates During mAb Purification

The Challenge

Have you seen two-peak elution of target monoclonal antibodies (mAb) after cation exchange (CEX) chromatography? The second elution peak consists of a mixture of monomers and aggregates of your target mAb and it leads to a decrease in your process yield.

Purification Tip

Choosing the optimal resin for your process will minimize on-column aggregates and maximize recovery. Be sure to screen multiple resins during your mAb purification process development.

Our Approach

On-column aggregate formation depends on the specific chromatography resin used. Resin structure, surface extenders, pore size and distribution each contribute to on-column aggregate formation. Bio-Rad’s Nuvia™ HR-S Resin — a strong cation exchanger with a pore size of 50 μm without surface extenders — minimizes on-column aggregates. Nuvia HR-S generates only 10% aggregate formation relative to competing resins under similar experimental conditions.