GORE® Protein Capture Devices: Increased Productivity At Multiple Scales With Rapid CIP

By Lauren Parker, Jeffrey Cassel, Mike McManaway, and William C. Barrett, Ph.D, W. L. Gore & Associates, Inc.

Cleaning during the Protein A step in antibody purification cycling is crucial as bioburden and endotoxin could be introduced to the system from the harvest feed stream or an external source, creating a risk of contamination.

Read through a demonstration of a rapid CIP being utilized and optimizing the loading and non-loading residence times during cycling to achieve increased productivity on a Gore Protein Capture Device. The method is easily transferred from lab scale to pilot scale sizes as demonstrated using both PROA101 (1mL) and PROA201 (58 mL) Gore Protein Capture Devices.