Getting The Most Out Of Cell-Based Assays With The Optimal Read Function For Fluorescence Bottom Measurements

Accurate fluorescence-based analysis of adherent cell lines often hinges on how well the measurement captures the true distribution of cells across a microplate. This application note introduces a whole-well analysis approach, Optimal Read (OR), that significantly improves sensitivity and reproducibility in bottom-read fluorescence measurements. By illuminating and measuring across the entire well, OR accounts for non-uniform cell growth, which is common in many cell types. Comparative data show that OR reduces measurement variation and delivers more realistic confluence assessments than traditional single-point reads. Especially in 48- and 96-well formats, this method enhances confidence in cell-based assay results.

For researchers working with heterogeneous cell layers, adopting whole-well illumination and analysis can lead to more reliable insights. Explore how OR can elevate your assay performance.