Get Reproducible Purification Scale-Up Results Faster With Prepacked Process-Scale Chromatography Columns

Three prepacked CHT Ceramic Hydroxyapatite Type I columns (40 μm particle size) were evaluated, ranging from 1.6 to 33 cm in inner diameter and 20 cm in bed height.

A protein mixture containing bovine serum albumin, lysozyme, and lactoferrin (0.8-1 mg/ml) was loaded onto each column, which was then equilibrated with 5 mM sodium phosphate and 10 mM NaCl buffer at pH 7.2.

The sample mixture was eluted at a flow rate of 100 cm/hr using a linear gradient of 5 mM sodium phosphate, 10 mM NaCl, pH 7.2, to 400 mM sodium phosphate, pH 7.2, over 10 column volumes. This was followed by a 3-column volume hold in the high phosphate buffer.

The Foresight Pro Column's innovative design and CHT Media packing ensure consistent purification performance across various column sizes. This reliability allows for efficient and scalable purification processes, streamlining your workflow.