Generate CHO-K1 GFP-Expressing Cells For Cell Line Development Workflow

Generating stable CHO-K1 cell lines expressing green fluorescent protein (GFP) is a critical step in many cell biology workflows, from imaging to functional assays. This white paper outlines a streamlined approach using the Uno Single Cell Dispenser™ to isolate and expand single CHO-K1 cells post-transfection. The method achieved a 93% single-cell isolation success rate and yielded 149 GFP-expressing colonies, with nearly 42% of dispensed cells forming viable clones. The process—from electroporation to stable clone verification—was completed in just over a month, with single-cell dispensing taking under 10 minutes. This workflow demonstrates how automation can improve efficiency and reproducibility in cell line development.

For labs seeking to accelerate clonal isolation while maintaining high fidelity, gain insight into a practical, data-backed solution.