GA4 Small Scale Gas Analyzer For Microbial Fermentation And Cell Culture Processes

This short protocol describes the function and application of Eppendorf’s 4-fold gas analyzer module GA4. The GA4 can be used in different processes with the Eppendorf small scale and modular systems together with the process control software DASware® control or as a standalone module.

The Eppendorf Off-Gas Analyzer GA4 as a process analytical tool provides information on important metabolic data in real time. It can be used in both microbial fermentation and cell culture processes. The GA4 provides separate discrete measurement channels for up to four bioreactors measuring oxygen and carbon dioxide content in the exhaust gas flow of the bioreactor. Critical process values, such as the oxygen transfer rate, carbon dioxide transfer rate, and the respiratory quotient are calculated using the integrated logic. These values give information about the respiratory activity of the organisms.