From Discovery To Verification: Unlocking miRNA Biomarkers With NGS And Digital PCR

Advancements in next-generation sequencing (NGS) have transformed transcriptome and small RNA profiling, enabling gene expression studies and the identification of splice variants, isoforms and novel transcripts from challenging samples like liquid biopsies, FFPE tumors and exosomes. This enhances our understanding of molecular mechanisms and aids in identifying RNA variants as potential disease biomarkers.

In this webinar, we will explore a complete workflow for miRNA biomarker discovery and verification using NGS and digital PCR (dPCR).

Learning objectives

• Set up for miRNA-seq success: Considerations for sample extraction and library preparation for miRNA-seq projects
• Analyzing your data: Bioinformatic pipeline for analysis
• dPCR assay design: How to leverage sequencing data to verify and design sensitive miRNA biomarker assays by dPCR
• Best practices for sequencing projects: Tips for engaging with sequencing cores