Fluorochrome Performance Guide

Designing effective flow cytometry panels requires more than familiarity with common dyes—it demands a clear understanding of fluorochrome properties. Differences in spillover and resolution can significantly impact data quality, making panel design complex. This guide simplifies the process by ranking fluorochromes based on spillover and stain index, helping users prioritize clean dyes for minimal spectral overlap. It also offers pairing recommendations based on antigen density, ensuring optimal resolution across diverse markers. With practical strategies and examples, including a 17-color panel design, this resource empowers researchers to reduce spread, improve clarity, and achieve high-quality multiparameter analysis. Explore how to streamline your panel design and maximize biological insights.