Fluorochrome And Laser Reference Guide: Explore The Full Spectrum Of BD Biosciences Flow Cytometry Fluorochromes

Understanding the optimal pairing of fluorochromes with lasers is essential for achieving accurate and high-quality flow cytometry results. This resource provides a comprehensive guide to the excitation and emission properties of a wide range of fluorochromes, including polymer-based, tandem, and small organic dyes. It highlights relative brightness levels, recommended antigen density pairings, and strategies to mitigate nonspecific binding for Cy dyes. Organized by laser type—ultraviolet, violet, blue, yellow-green, and red—it offers practical insights for selecting fluorochromes that minimize spillover and maximize resolution. Whether working with low-density surface markers or high-density intracellular targets, this reference helps streamline panel design and improve experimental consistency. Explore the full spectrum to optimize your instrument configuration and staining strategy.