Fluorescent Western Blot Detection

This handbook offers a comprehensive guide to fluorescent Western blot detection, optimized for use with Odyssey® imaging systems. It covers every step from membrane preparation to imaging, with detailed instructions for both nitrocellulose and PVDF membranes. Gain insight into the best practices for antibody handling, detergent use, and equipment cleanliness to minimize background and maximize signal clarity, as well as guidelines for multiplexed detection to help researchers design experiments that detect multiple targets simultaneously. Special attention is given to optimizing antibody concentrations and adapting protocols for different imaging channels.

Whether you're troubleshooting or refining your workflow, find out how you can improve consistency and sensitivity.