Fluorescence Vs. UV Detection: Comparing Two CE-SDS Systems Using NISTmAb

Fluorescence and UV detection are both widely used in protein capillary electrophoresis, but how do they compare in real analytical workflows? Side-by-side evaluation using the same monoclonal antibody reference standard reveals strong alignment between detection approaches, with both generating consistent, reproducible data. Results closely match established reference values, reinforcing confidence in accuracy regardless of detection choice. By examining performance across two distinct capillary electrophoresis systems—one fluorescence-based and one UV-based—key similarities in data quality and analytical reliability come into focus. For teams optimizing methods or evaluating instrumentation, these findings offer practical insight into selecting a detection strategy without sacrificing confidence in results.

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