Filtration Of Cell Culture Growth Media And Process Buffers

Media feeds and process buffers are two universal additives to biopharmaceutical processes. Media feeds are added to a fermenter to facilitate growth of mammalian, yeast, insect, and bacterial cells. Process buffers are used in conjunction with chromatography columns, for diafiltration and as a general solvent for protein solutions. Media feeds and process buffers may be formulated on site or purchased in pre-filled disposable packaging.

Media feeds and process buffers require filtration to protect against the introduction of bacteria, viruses, endotoxins and particulates that can contaminate and compromise processes. This 3M Purification Application Brief presents several alternatives for filtration of media feeds and buffers.

Media feeds consist of cell culture growth supplements added to the fermentation vessel in bulk for batch processes or continuously in perfusion processes. Depending on the process, there may be more than one type of media feed added. Media feeds can be produced on site by mixing each of the various components in a media mix tank, followed by filtration prior to addition to the fermenter. If growth media is pumped directly from the media mix tank to the fermenter, a vent filter is required on the media mix tank to prevent ingress of bacteria. Growth media can also be purchased in pre-filled disposable packages.