Fast And Reproducible AAV Vector Quantification With Simple Plex Assays On Ella

As part of the viral quantification process, researchers rely on various analytical methods to assess critical quality attributes. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. To meet increased throughput and safety demands, fast and reproducible titration of viral particles is essential. This growing need has spurred the development of more automated analytical solutions, which are paving the way for increases in facility throughput while helping to make quality control faster, more accurate, and more reproducible.

Follow us through the process and results of pairing Simple Plex™ microfluidic cartridge assays on Ella™ with PROGEN antibodies to demonstrate utility for accurate and efficient AAV capsid titer determination. We will explore AAV spiked recovery, sample linearity of multiple in-process pools, and Simple Plex Assay precision.