Fed-batch culture is the commonly adopted biologicals manufacturing method used in many biopharmaceutical companies as it can generate gram/litre amounts of protein-based therapeutics. However, to obtain the maximum production of therapeutic proteins requires the optimum formulation of culture media and feeds, as well as the correct bioprocess parameters. The ambr 250 mini bioreactor system provides a new way of rapidly evaluating these bioprocess conditions to support efficient fed-batch culture.