Evaluation Of TFF Operating Control Strategies And Scalability For Viral Vector Process Development

Viral vector process development demands operating strategies that balance throughput, robustness, and scalability, especially in downstream concentration and diafiltration steps. Explore how two common tangential flow filtration control approaches, transmembrane pressure (TMP) control and permeate control, perform across representative viral vector workflows. Using both virus model feeds and AAV2 material, the analysis compares flux behavior, processing time, pressure stability, yield, and impurity clearance under varying membrane cutoffs and feed loadings. The results highlight clear tradeoffs between speed and impurity removal, while also demonstrating predictable scale-up from small-format cassettes to larger single-use systems. For teams designing or optimizing viral vector UF/DF operations, the findings offer practical guidance on selecting control strategies that align with performance goals and manufacturing scale.

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