Evaluation Of Signal Peptides For Enhanced Production Levels In CHO DG44 Cells

Source: Sartorius

By Nico Erb, Juliana Schubert, Caroline Hauser and Christoph Zehe

The high-titer production of biopharmaceuticals using “Chinese hamster ovary” (CHO) cells is an important pillar of the pharmaceutical industry. Nevertheless, a potential bottleneck for profitable production is the successful secretion of the synthesized proteins through the endoplasmatic reticulum (ER) and the Golgi apparatus into the culture medium directed by the signal peptide (SP). Since natural SP sequences and efficiencies vary between proteins and species, we demonstrated that the utilization of different SPs can lead to enhanced protein expression in already existing production systems.

access the Poster!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Bioprocess Online