Evaluation Of Protein Capture Devices Used In-Series For Monoclonal Antibody Purification

By Jared Clinger, B.S. and William C. Barrett, Ph.D., W.L. Gore & Associates, Inc.

The GORE Protein Capture Devices provides increased purification capacity at a short residence time, thus allowing the purification of over 30 to 105 mg of antibody in less than 40 minutes per purification cycle. In this paper we demonstrate how two 3.5 mL GORE Devices can be connected in-series, which doubles the column capacity by providing a 7.0 mL bed volume and allows capture of at least 200 mg of monoclonal antibody within 60 minutes.