Evaluating Clone Performance And Cell-Specific Productivity: Comparing The Cydem VT System And 10 L Bioreactor Cultivations

By Simon Briel and Sameera Qureshi

This content is brought to you by Beckman Coulter, a Danaher Operating Company.

Selecting top-performing clones requires more than endpoint measurements. Continuous monitoring of pH, dissolved oxygen, and biomass, combined with automated at-line analysis of total cell density, viable cell density, and IgG titer, provides a clearer view of clone performance and process consistency. The Cydem VT Automated Clone Screening System uses individually controlled microbioreactor wells, calibrated optodes, and PID-controlled gas delivery to maintain stable conditions for mammalian cell growth and antibody production. Scheduled feeding, base addition, and analytical testing further support consistent cultivation and data collection.

Explore how five CHO cell line clones were evaluated and ranked by comparing performance in the automated screening system with results from a 10 L bioreactor, providing insight into clone selection and scale-up.