Enzyme Substrates
These innovative substrates represent the next generation of X-Gal, X-Gluc and X-Phos, and are analogous to their chemical relatives in their usage. An advantage of these stains is their use in multi-staining applications with other chromogenic substrates. Other uses are: Detection of histochemical marker genes in cloning techniques; rapid and specific identification of E. Coli; localizing enzyme activity in tissue sections; and as sensitive indicators in enzyme-linked applications.
Red-Gal acts as a specific indicator of E-Coli's ß-galactosidase gene (LacZ) when used as a marker gene. Red-Gluc detects ß-glucuronidase in cloning methods that use the GUS gene fusion system.
We also offer common substrates, such as X-Gal, X-Gluc, MUG and pNPP, and have included unique substrates, such as X-a-Gal and X-a-Glu, for the detection of a-glycosidases. The novel substrate XPP (X-PPhos; 5-Bromo-4-Chloro-3-Indolyl Phenyl Phosphonate, p-Toluidine Salt) has been developed for the detection of the conditional lethal marker enzyme, phosphonate monoester hydrolase, utilized in the study of plant genetics.
We also offer a variety of enzymatic stains such as AEC, DAB and tetrazolium salts. Enzyme substrates fluorescein-di-ß-D-galactoside, 3-O-methyl fluorescein phosphate and carboxyfluorescein diacetate generate a fluorescent signal as a result of enzymatic activity. We also have introduced substrates for unique proteases that generate a fluorescent signal based on 7-Amino-4-Trifluoromethyl Coumarin.
Research Organics, Inc., 4353 East 49th St., Cleveland, OH 44125. Tel: 216-883-8025; Fax: 216-883-1576.