Enhancing Cell Line Development With Rapid Whole-Well Imaging Using The Tecan Spark® Cyto Multimode Reader

Efficient identification of optimal cell clones is essential for advancing biologics production and cellular research. This application note outlines a streamlined workflow using whole-well imaging with the Spark Cyto multimode reader to accelerate cell line development. By integrating CRISPR-based gene tagging, single-cell sorting, and automated imaging, researchers can rapidly evaluate clone viability and performance in 96-well plates. The Spark Cyto’s imaging capabilities—paired with its Image Analyzer software—enable high-throughput, reproducible clone selection while minimizing manual effort and error. The method supports longitudinal monitoring of clone growth and phenotypic traits, offering valuable insights for drug discovery and live-cell imaging applications.

Access the full application note to see how imaging-driven analysis can enhance your cell line development strategy.