Enhanced Sensitivity For Peptide Quantification In A Complex Matrix Using High-Resolution LC-MS/MS

By Shane Needham, Eshani Nandita, Lei Xiong, Elliott Jones, Zoe Zhang, and Kerstin Pohl

A significant 5-fold improvement in LLOQ for peptide quantification was achieved using the ZenoTOF 7600 system featuring the Zeno trap. Compared with traditional time-of-flight systems, the Zeno trap enables greater MS/MS sensitivity by enhancing the duty cycle.

High-resolution platforms, such as traditional time-of-flight systems, often lack sensitivity due to loss of ion transmission in between TOF pulses. This application note will explore the key features of the ZenoTOF 7600 system for highly sensitive peptide quantification and the methods for which it can be used.