Enhanced Biotherapeutic Protein Expression Using Advanced Vector Systems

In the fast-paced field of recombinant protein manufacturing using GS-CHO cells, it is essential to begin with an optimized expression vector delivered into the host cell line during transfection. Expression vector design involves numerous variables that can significantly impact final product titre, product quality, and long-term expression stability; among these, the choice of promoter driving transcription of the product genes is particularly critical.

This presentation outlines the development of Lonza’s GSquad® Pro expression vector platform, featuring the engineered high-performance promoter LHP-1, which provides notable improvements across key biomanufacturing performance metrics. It also offers scientific insights into how Lonza is generating industry-leading datasets on gene expression control in CHO cells and applying these findings to refine DNA vector design.

Viewers will gain an understanding of the key design elements of CHO expression vectors, explore the performance advantages of the GSquad® Pro platform, and learn about emerging data-driven strategies for enhancing gene expression control in CHO systems.