Enabling Accelerated Raman Model Calibration For Seamless And Reproducible Real-Time Monitoring By Combining Raman And Automated Sampling Technologies

Raman spectroscopy is a key component of a process analytical technology (PAT) strategy in bioprocessing to manage the performance of the process and ensure the quality of the final product. However, Raman calibration necessitates operators to manually collect samples, which increases the risk of bioreactor contamination and consumes significant time and resources.

This study evaluates an innovative workflow designed to streamline the integration of an in-line Raman analyzer for bioreactor applications. The workflow uses on-line technology for automated sampling and data analysis, incorporating the ProCellicsTM Raman Analyzer and the MAST® Autosampling Solution. This approach accelerates the Raman model-building phase and enables real-time, in-line monitoring of critical quality attributes (CQAs) and critical process parameters (CPPs) during manufacturing.