Enable The Elution Of Fc-Containing Molecules At Higher pH Levels With Praesto® Jetted A50 HipH

By Mark Hicks, Benjamin Summers, Rosie Jackson, and Hans Johansson

With the current advancement of antibody engineering and drug development, new antibody types such as Fc-fusion proteins and other antibody-derived drugs are emerging. This type of molecule is commonly more sensitive to acidic conditions and will in some cases aggregate when typical protein A elution buffers are used. For large-scale purification of antibodies and Fc-fusion proteins, protein A affinity chromatography is used in the capture step for almost every established purification platform.

In this poster, you can analyze application data from the use of a new protein A resin, Praesto Jetted A50 HipH, which enables elution of Fc-containing molecules up to pH 5.0. Some of the key features include:

• Mild elution – up to pH 5.0.
• Up to 60 mg/ml dynamic binding capacity for polyclonal human igG.
• Uniform jetted beads – efficient mass transfer.
• Resistant to 0.1 M NaOH for >100 cycles (15 min contact time).