Product/Service

Elisa Kits--Human IL10 Elisa Kit

Source: Cell sciences, Inc.
The IL-10 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA)Cell sciences, Inc.
Catalog No. 850.060.096 1 x 96 tests

PRINCIPLE OF THE METHOD

The IL-10 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated monoclonal antibody specific for IL-10‘ are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen.

SPECIFICITYNo cross reactivity with other cytokines

PERFORMANCES

  • Sensitivity < 5 pg/mL

  • Range 12.5 pg/mL-400pg/mL

  • Sample Serum, Plasma, or cell-culture supernatants

  • Sample Volume 100µl

  • Calibration WHO 92/516

  • Linearity of Dilution A human serum pool containing 400 pg/ml of measured IL-10 was serially diluted in standard buffer diluent over the range of the assay. Linear regression of samples versus the expected concentration yielded a correlation coefficient of 0.99.

  • Recovery Recovery of IL-10 added to pooled normal serum is 96.2% (89 % to 99.9%) for IL-10 concentrations ranging from 400 to 15 pg/ml

    Cell sciences, Inc., 1504 Providence Highway, No: 11B, Norwood, MA 02062. Tel: ; Fax: 781-769-0542.