Efficient Purification Of A Recombinant Bacterial DyP-Type Peroxidase With A Hydrophobic Anion Exchange Resin

DyP (dye-decolorizing peroxidase) -type peroxidases are a family of heme-containing enzymes. They are capable of catalyzing efficient oxidation of a wide array of industrially relevant substrates including dyes with anthraquinone structure, β-carotene, and aromatic sulfides. Owing to their remarkable expression and robustness, they have many potential uses in a variety of biotechnology applications. Here we show polish purification of bacterially expressed DyPs with a hydrophobic anion exchange – mixed mode resin – Nuvia aPrime 4A, to generate a >95% pure, functionally viable sample. The two-step workflow used in this purification is readily scalable for process production.