Effect Of Column Chemistry, Temperature, Inert Flow Path, And Mobile Phase On The Separation Of GLP-1 Peptides And Their Impurities

As GLP-1 therapies move into broader development and production, impurity profiling is becoming more demanding. Peptide-related impurities can be difficult to separate because they may share nearly identical molecular masses and closely matched chemical properties, leaving little room for error in LC-UV and LC/MS workflows. Effective method development depends on understanding how mobile-phase composition, additive choice, column chemistry, temperature, and sample loading affect resolution and sensitivity. Key findings show how TFA can improve peak shape and impurity separation compared with formic acid, while temperature optimization can sharpen selectivity for difficult peptide pairs. Charged C18 stationary phases also offer advantages in peak capacity and loading tolerance.

Access the full poster to learn how these method variables can support more reliable GLP-1 impurity characterization.