DNA Clean & Concentrator-5

Highlights

• Ultra-pure DNA in 2 minutes: Add DNA and Binding Buffer to column, spin, wash, and elute.
  
• Clean-up and concentrate DNA samples from PCR reactions, restriction enzyme digestions, crude homemade minipreps and any source of DNA.
  
• For the most optimal biological reactions, high quality sequencing, efficient ligations, restriction enzyme digestion, transcriptions, labeling and any reactions that require high quality DNA.

General Description
DNA Clean & Concentrator-5™ efficiently cleans-up and concentrates DNA samples from PCR reactions, restriction enzyme digestions, and low concentration of DNA or "dirty" DNA to 6 ul in pure water in just two minutes. Simply apply your DNA sample and supplied DNA Binding Buffer to the column, then spin, wash and elute the DNA with water. That's all. No ethanol precipitation. No phenol. No chloroform. The kit is based on our Zymo-Spin column that features very small, ~6 ul, final DNA recovery volume and user-friendly single DNA Binding Buffer. The high quality DNA recovered is especially good for the most efficient down stream DNA manipulations such as ligations, restriction enzyme digestions, sequencing, RNA transcriptions, transfections, E. coli and yeast transformations, and probe labeling.

Protocol
Before starting: Add 16 ml (64 ml for 200 DNA clean-up & concentrations of product D4004) of 100% ethanol to the Wash Buffer Concentrate to make final Wash Buffer.

1. Add 2 volumes of DNA Binding Buffer* to each volume of DNA sample.
Example: 150 ul DNA sample, add 300 ul of DNA Binding Buffer.
For samples less than 50 ul, simply add 100 ul of DNA Binding Buffer to the sample, then proceed.
Example: 25 ul DNA from restriction enzyme digestion, add 100 ul DNA Binding
Buffer.
2. Load sample into a Zymo-Spin Column and place column into a 2 ml collection tube.
3. Centrifuge at full speed (>10,000 g) for 5-10 seconds. Discard the flow-through.
The capacity of the column is 600 ul. Load twice if the volume is more than 600 ul. The capacity of the collection tube is 600 ul with the column inserted. Empty collection tube whenever necessary if the volume is more than 600 ul to prevent the contamination of column by flow-through.
4. Add 200 ul of Wash Buffer to the column. Spin at top speed for 5-10 seconds. Add another 200 ul of Wash Buffer. Spin at top speed for 30 seconds.
Important! A longer spinning duration for this last wash is necessary for complete removal of wash buffer residues.
5. Add 6-8 ul of water directly to the column matrix. Place column into a 1.5 ml tube. Spin briefly to elute the DNA.
Note: TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) can also be used for the elution step instead of water. Waiting for one minute after adding water or TE before centrifuging can improve DNA recovery rate for larger sizes of DNA (>6 Kb).

DNA is now ready for your experiments.

*Contains Chaotropic reagents. Irritant. Please use proper safety precautions with this reagent.

General Information

Specificity
DNA Purity: Ultra-pure DNA is recovered in water which is especially good for ligations and sequencing. The DNA can also be used for restriction enzyme digestion, transcriptions, labeling and any reactions that require high quality DNA.
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Recovery Volume: 6-8 ul

DNA Size: 75 bp - 23 Kb

Detergent Tolerance: Up to 5% Triton X100, 5% Tween-20, 5% Sarkosyl, 0.1% SDS

Recovery Rate: 75 bp - 10 Kb: 70-90% 11 - 23 Kb: 50-70%

DNA Binding Capacity: 5 ug

Stability: Quality is guaranteed for 1 year from the purchase date.

DNA Sources: All restriction enzyme digestion reactions. PCR reactions, homemade minipreps, alkaline phosphatase reactions, kinase reactions, different PCR reactions, etc.

Zymo Research, 625 West Katella Ave, Suite 32, Orange, CA 92867-4619. Tel: 714-288-9682; Fax: 714-288-9643.