Direct Amplification Of Cell Culture Samples

This content is brought to you by Integrated DNA Technologies, a Danaher Operating Company.

Accelerating qPCR workflows often hinges on eliminating bottlenecks, and nucleic acid extraction is a major one. Direct amplification of cell culture samples offers a streamlined alternative, enabling researchers to move from sample collection to analysis without the typical purification step. Using a broad-range one-step master mix, cell samples collected via trypsinization, lysis buffer, or simple scraping methods demonstrate comparable amplification performance to normalized controls across multiple mRNA targets. This approach not only preserves data quality but can also reduce turnaround time by roughly two hours while lowering per-sample costs. By simplifying preparation and maintaining reliable results, direct amplification supports faster experimental cycles and more efficient lab operations.

Explore how this workflow can help optimize your qPCR processes and increase throughput without added complexity.