Webcast: Development Of IEX Purification Process For Lentiviral Vectors

Review data presented on a high-throughput screening strategy that was employed for LVV purification which provided high yield as well as acceptable HCP clearance.

An initial high-throughput screening experiment was carried out to broadly evaluate anion exchange resins that exhibited high LVV recovery. LVV stability was studied in parallel, and conditions were established where the vector exhibited good stability. A second screening was then carried out with a subset of resins selected from the first screen under stable conditions to identify optimal wash and elution steps to further improve the product yield and protein clearance. The large set of data provided in this work represents an important contribution to knowledge in the field about the chromatographic efficacy of a wide range of resins for LVV bioprocessing under stable conditions.